Andrzej Joachimiak

Director of Structural Biology Center and Midwest Center for Structural Genomics
- Biosciences

Andrzej Joachimiak is an expert in synchrotron-based X-ray crystallography and structural biology.  At Argonne, he has made significant contributions to the high-throughput crystallography using synchrotron radiation and the development of state-of-the-art facilities for macromolecular crystallography. The development and integration of the novel synchrotron beamlines, exploitation of the anomalous signal-based phasing methods in the third-generation environment and integration of hardware and software at the Structural Biology Center beamlines at the Advanced Photon Source contributed very strongly to the enhanced efficiency of such facilities worldwide.

Andrzej’s research has also contributed to methods development in protein expression and purification, crystallization and data collection using synchrotron radiation. The methods for phasing novel structures using X-ray crystallography have effectively reduced time and cost of structure determination. The contribution to high-throughput molecular biology and crystallography has also made a major impact on structural biology in the U.S. and globally as well. This encouraged the initiation of structural genomics and structural proteomics projects and also led to the determination of thousands of novel protein structures.

He has also made highly important contributions to the field of structural genomics. The Argonne-based Midwest Center for Structural Genomics (MCSG) is a highly successful program and major component of NIH funded Protein Structure Initiative (PSI). A number of technologies have been developed in the MCSG, including gene cloning and protein expression, new vectors for protein expression in bacteria, automated protein purification techniques, automated protein crystallization, robot-assisted crystal mounting and automated structure determination.

Andrzej’s current research also focuses on proteins and protein-nucleic acid interactions and includes enzymes, transcription factors and molecular chaperones.

In addition to his duties at Argonne, Andrzej is also a Professor at the University of Chicago, Adjunct Professor at Northwestern University, Senior Fellow at the  Computation Institute  and the Institute for Genomics and Systems Biology.

Awards, Honors and Memberships

  • Fellow of the American Association for the Advancement of Science
  • Member of the American Crystallographic Association
  • Member of Protein Society
  • University of Chicago Award for Distinguished Performance
  • Member European Academy of Sciences
  • Member International Structural Genomics Organization Advisory Board
  • Member Protein Data Bank Advisory Committee
  • Arthur H. Compton Award, Advanced Photon Source
  • Member Polish Academy of Arts and Sciences

Selected Publications (from 275 total)

  • Kim, Y.C., Quartey, P., Li, H., Volkart, L., Hatzos, C., Chang, C., Nocek, B., Cuff, M., Osipiuk, J., Tan, K., Fan, Y., Bigelow, L., Maltseva, N., Wu, R., Borovilos, M., Duggan, E., Zhou, M., Binkowski, T.A.,  Zhang, R. and Joachimiak, A. A Large-Scale Evaluation of Protein Reductive Methylation for Improving Protein Crystallization. (2008) Nat Methods. 5:853-4.
  • Joachimiak, A, High-throughput crystallography for structural genomics (2009) Cur. Opi. in Str. Biol. 19:573-84.
  • Wu, R, Richter S, Zhang RG, Anderson VJ, Missiakas D, Joachimiak A. Crystal Structure of Bacillus anthracis Transpeptidase Enzyme CapD. (2009) J Biol. Chem. 284:24406-14
  • Petrova, T, Ginell S, Mitschler A, Kim YC, Lunin VY, Joachimiak G, Cousido-Siah A, Hazemann I, Podjarny A, Lazarski K, Joachimiak A. X-ray induced deterioration of disulfide bridges at atomic resolution (2010), Acta Cryst. D, 66:1075-1091.
  • Tan, K, Tesar C, Wilton R, Keigher L, Babnigg G, Joachimiak A. Novel {alpha}-glucosidase from human gut microbiome: substrate specificities and their switch.  (2010) The FASEB Journal, 24:3939-49.
  • Finfrock, YZ, Stern E.A, Yacoby Y, Alkire RW, Evans-Lutterodt K, Stein A, Isakovic AF, Kas JJ, and  Joachimiak A. Spatial dependence and mitigation of radiation damage by line-focus mini-beam, (2010) Acta. Cryst. D. 66:1287-1294.
  • Kim, Y, Joachimiak G, Ye Z, Binkowski TA, Zhang R, Gornicki P, Callahan SM, Hess WR, Haselkorn R, Joachimiak A. Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria. (2011) Proc Natl Acad Sci U S A. 108:10109-14.
  • Kim, Y, Tesar C, Mire J, Jedrzejczak R, Binkowski A, Babnigg G,  Sacchettini J, and Joachimiak A, Structure of Apo- and Monometalated Forms of NDM-1 – a Highly Potent Carbapenem-Hydrolyzing Metallo-β-Lactamase, (2011) Plos One, 6:e24621. (PMID: 21931780).
  • Kim Y, Babnigg G, Jedrzejczak R, Eschenfeldt WH, Li H, Maltseva N, Hatzos-Skintges C, Gu M, Makowska-Grzyska M, Wu R, An H, Chhor G, Joachimiak A. High-throughput protein purification and quality assessment for crystallization.  (2011) Methods. 55:12-28 (PMID: 21907284).
  • Osipiuk J, Mulligan R, Bargassa M, Hamilton JE, Cunningham MA, Joachimiak A. Characterization of Member of DUF1888 Protein Family, Self-cleaving and Self-assembling Endopeptidase. J Biol Chem. 2012 Jun 1;287(23):19452-61. Epub 2012 Apr 5. PubMed PMID: 22493430; PubMed Central PMCID: PMC3365983.  
  • Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A. Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from Bacillus anthracis. J Mol Biol. 2012 Apr 16. [Epub ahead of print] PubMed PMID: 22516613.
  • Yang DC, Tan K, Joachimiak A, Bernhardt TG.  A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division.  Mol Microbiol. 2012 Jun 20. doi: 10.1111/j.1365-2958.2012.08138.x. [Epub ahead of print]  (PMID: 22715947).
  • Michalska K, Chang C, Mack JC, Zerbs S, Joachimiak A, Collart FR. Characterization of Transport Proteins for Aromatic Compounds Derived from Lignin: Benzoate Derivative Binding Proteins. J Mol Biol. 2012 Aug 25. [Epub ahead of print] PubMed PMID: 22925578.  
  • Binkowski TA, Marino SR, Joachimiak A. Predicting HLA Class I Non-Permissive Amino Acid Residues Substitutions. PLoS One. 2012;7(8):e41710. Epub 2012 Aug 8. PubMed PMID: 22905104; PubMed Central PMCID: PMC3414483.