Compressive Reconstruction of 3D Snapshot Imaging with Multifocal Plane Microscopy
Abstract: In this talk, I will present a compressive sensing imaging system capable of measuring a three-dimensional (3D) dynamic fluorescent scene of approximately 20x20x10-µm range in 100x100x50-nm resolution at 25 frames per second. Classic 3D imaging systems such as confocal microscopy require lengthy z-scans, thus fail to capture moving objects. Our system does not need z-scans but rather utilizes multifocal plane microscopy to instantly capture a two-dimensional (2D) image that encodes the 3D object’s information. From the depth-encoded 2D image, we developed a compressive sensing technique to recover the corresponding 3D object. In the end, I will talk about four-dimensional joint spatial-temporal reconstruction and its relationship to 3D tomography with uncalibrated rotation and translation.