Photophysical Considerations in Developing Fluorescent Indicators for Zinc Ions
The significance of developing fluorescent indicators targeting zinc(II) ions arises from the intimate involvement of zinc(II) ions in many biological processes. On the fundamental front, zinc(II) indicator research has enriched the knowledge base of the photophysical processes of organic fluorophores. In this presentation, several classes of synthetic indicators for zinc( II) ions that have been developed in our laboratory are described, with an emphasis on delineating how coordination with the redox-innocuous zinc(II) ions changes the fluorescence of the organic ligand. A special focus is placed on the compounds that undergo intramolecular zinc(II)-coordination dependent Forster resonance energy transfer (FRET). T he photophysical characterization using femtosecond time-resolved transient absorption spectroscopy and the applications of selected compounds in confocal and structured illumination spectroscopies are demonstrated.
About the Presenter:
Lei Zhu received his BS Degree in chemistry from Peking University in China in 1997. Subsequently, Lei went to New York University for graduate studies in chemistry under the mentorship of Professor James Canary. After defending his PhD dissertation in January 2003, Lei joined Professor Eric Anslyn's group at the University of Texas at Austin as a postdoctoral fellow. Upon completing his postdoctoral appointment, Lei started his independent career as an Assistant Professor in the Department of Chemistry and Biochemistry at Florida State University in August 2005. He was promoted to the rank of Associate Professor in 2011.