This invention describes a system for crystallization and data collection of proteins using fixed-target serial crystallography using microfluidic droplet crystallography for the generation of uniform small crystals and a nylon mesh holder for positioning of droplets with crystal (or batch crystal slurries). While the use of nylon has previously been reported in the literature, this invention includes a method and device design for sealing the droplet/slurry loaded mesh between Mylar sheets.

3D printing has been used to print an extremely thin layers of material in a pattern with specifically designed wells to hold and immobilize crystals. These wells can vary in size and shape which increases the flexibility of this invention for use in different experimental modes. A key benefit for this invention is the ability to print a mesh directly onto mylar or other sealing materials.

The invention describes a new sample holder for serial crystallography that utilizes a magnetic compression ring to immobilize sample fluids between mesh. Advantages of the new technology include, compatibility with standard single crystallography mounting equipment, 3D printable, minimal assembly required, high reusability, and cost effective.