Andrzej Joachimiak

Dr. Andrzej Joachimiak is an expert in synchrotron-based X-ray crystallography and structural biology.  At Argonne, he has made significant contributions to the high-throughput crystallography using synchrotron radiation and the development of state-of-the-art facilities for macromolecular crystallography.   Dr. Joachimiak has been studying structures of proteins and their complexes, including protein/small ligand complexes for the past 40 years.   His expertise is in protein structure and function, molecular basis of diseases, enzyme catalysis, protein/protein, protein/small ligands and protein/nucleic acid interactions.  He uses these approaches combined with biochemical and functional analyses to study biological systems at the atomic level.  For the past 30 years, he served as the Director of Structural Biology Center, one of the world’s leading facilities for protein structure determination.  In the past 24 years, the NIH has supported his research in this area as a part of the Protein Structure Initiative, the Regional Center of Excellence, and the Structural Genomics/Biology of Infectious Diseases programs. He has been involved in several large and highly successful collaborative efforts to study proteins, enzymes and drug discovery relevant to human diseases, including cancer and more recently SARS-CoV-2. His laboratory contributed many high-resolution crystal structures of viral proteins and complexes with interacting partners, antibodies and small ligands.

Andrzej’s research has also contributed to the field of structural genomics.  The Argonne-based Midwest Center for Structural Genomics (MCSG) was a highly successful program and major component of NIH funded Protein Structure Initiative that developed methods in protein expression and purification, crystallization and data collection using synchrotron radiation. The methods for phasing novel structures using X-ray crystallography have effectively reduced time and cost of structure determination. The contribution to high-throughput molecular biology and crystallography has also made a major impact on structural biology in the U.S. and globally as well. This encouraged the initiation of structural genomics and structural proteomics projects and also led to the determination of thousands of novel protein structures and have been successfully applied to emerging diseases.

In addition to his duties at Argonne, Andrzej is also a Professor and Senior Scientist of the Consortium for Advanced Science and Engineering  at the University of Chicago, Adjunct Professor and Senior Fellow at the Institute of Science and Engineering at Northwestern University, CoPI, DOE Biopreparedness Research Virtual Environment, Taskforce 5. 

Education
•    B.S/M.S. Molecular Biochemistry, University of A. Mickiewicz of Poznan 
•    Ph.D., Chemistry, University of A. Mickiewicz of Poznan
•    Postdoc, Biophysics, University of Chicago, IL    
•    D. Sc., Molecular Biology, Institute of Biochemistry and Biophysics, Warsaw

Awards, Honors and Memberships
•    Fellow of the American Association for the Advancement of Science
•    Member of the American Crystallographic Association
•    Member of Protein Society
•    University of Chicago Award for Distinguished Performance
•    Member European Academy of Sciences
•    Member International Structural Genomics Organization Advisory Board
•    Member Protein Data Bank Advisory Committee
•    Arthur H. Compton Award, Advanced Photon Source
•    Member Polish Academy of Arts and Sciences
•    DOE BER Advisory Committee, Member
•    Polish Synchrotron Radiation Society, Member
•    CASP Advisory Board, Member
•    University of Chicago Ricketts Laboratory IBC, Member

Citations
H-Index: 95, Citations: 38,363 
Full list of publications on Google Scholar.

Publications (peer reviewed selected)
•    Schevitz R, Otwinowski Z, Joachimiak A, Lawson C, Sigler P. Three-dimensional structure of the trp repressor. (1985) Nature 317:782-786. PMID: 3903514
•    Joachimiak A, Marmorstein R, Schevitz R, Mandecki W, Fox R, Sigler P.  Crystals of the trp repressor-operator complex suitable for X-ray diffraction analysis. (1987) J. Biol. Chem. 262:4917-4921. PMID: 3549711 
•    Otwinowski Z, Schevitz R, Zhang R, Lawson C, Joachimiak A, Marmorstein R, Luisi B, Sigler P. The crystal structure of trp repressor/operator complex at atomic resolution. (1988) Nature, 335:321-329.
•    Braig K, Otwinowski Z, Hegde R, Boisvet D, Joachimiak A, Horwich A, Sigler P. The crystal structure of the bacterial chaperonin GroEL at 2.8 Å, (1994) Nature, 371:578-586.
•    Joachimiak A, Haran T, Sigler P.  Mutagenesis supports water mediated recognition in the trp repressor/operator system. (1994) EMBO J., 13:367-372. PMID: 8313881
•    Thiyagarajan P, Henderson S, Joachimiak A.  Solution structure of GroEL and its complex with rhodanese from small angle neutron scattering. (1996) Structure, 4:79-88. PMID: 8805508
•    Joachimiak A.  Capturing the misfolds: chaperone-peptide-binding motifs. (1997) Nature Structural Biol. 4:430-434.
•    Sriram M, Osipiuk J, Freeman B, Morimoto R, Joachimiak A. Human 70 molecular chaperone binds two calcium ions within the ATPase domain, (1997) Structure 5:403-414. PMID: 9083109
•    Xiong J, Stehle T, Diefenbach B, Zhang R, Dunker R, Scott D, Joachimiak A, Goodman S, Arnaout A. Crystal structure of Integrin aVb3. (2001) Science, 294:339-45. PMID: 11546839
•    Xiong J, Stehle T, Zhang R, Joachimiak A, Goodman S, Arnaout M.  Crystal structure of integrin aVb3 in complex with an Arg-Gly-Asp ligand (2002) Science, 296:151-155
•    Zhang R, Pappas T, Brace J, Miller P, Oulmassov T, Molyneaux J, Anderson J, Bashkin J, Winans S, Joachimiak A. Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. (2002) Nature 417:971-4. PMID: 12087407 
•    Li D, Zhao R, Lilysrtom W, Gai D, Zhang R, Decaprio J, Fanning, E, Joachimiak A, Szakonyi G, Chen X.  Structure of the replicative helicase of the oncoprotein SV40 large tumor antigen. (2003) Nature 423:512-518. 
•    Mazmanian S, Skaar E, Gaspar A, Humayun M, Gornicki P, Jelenska J, Joachimiak A, Missiakas D, Schneewind O.  Passage of heme-iron across the envelope of Staphylococcus aureus. (2003) Science 299:906-9.
•    Howard EI, Sanishvili R, Cachau RE, Mitschler A, Chevrier B, Barth P, Lamour V, Van Zandt M, Sibley E, Bon C, Moras D, Schneider TR, Joachimiak A, Podjarny A. Ultrahigh resolution drug design I: Details of interactions in human aldose reductase-inhibitor complex at 0.66 Å. (2004) Proteins. 55:792-804.
•    Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. (2006) Science. 312:1526-30. (PMC2800167). 
•    Shen Y, Joachimiak A, Rosner MR, Tang WJ. Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. (2006) Nature, 443:870-4.
•    Tan K, Tesar C, Wilton R, Keigher L, Babnigg G, Joachimiak A. Novel {alpha}-glucosidase from human gut microbiome: substrate specificities and their witch.  (2010) The FASEB Journal, 24:3939-49.
•    Donnelly MI, Zhou M, Millard CS, Clancy S, Stols L, Eschenfeldt WH, Collart FR, Joachimiak A. An expression vector tailored for large-scale, high-throughput purification of recombinant proteins. (2006) Protein Exp. & Pur.,47:446-54. PMC2792030.
•    Kim YC, Quartey P, Li H, Volkart L, Hatzos C, Chang C, Nocek B, Cuff M, Osipiuk J, Tan K, Fan Y, Bigelow L, Maltseva N, Wu R, Borovilos M, Duggan E, Zhou M, Binkowski TA, Zhang R, Joachimiak A. A large-scale evaluation of protein reductive methylation for improving protein crystallization. (2008) Nat Methods. 5:853-4. PMC2678869.
•    Kim YC, Zhou M, Moy S, Morales J, Cunningham MA, Joachimiak A. Structure and analysis of the nitrile reductase QueF from V. cholera.  (2010) J. Mol. Biol. 404:127-137.
•    Babu M, Beloglazova N, Flick R, Graham C, Skarina T, Nocek B, Gagarinova A, Pogoutse O, Brown G, Binkowski A, Phanse S, Joachimiak A, Koonin EV, Savchenko A, Emili A, Greenblatt J, Edwards AM, Yakunin AF. A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. (2011) Mol Microbiol. 79:484-502.
•    Kim Y, Joachimiak G, Ye Z, Binkowski TA, Zhang R, Gornicki P, Callahan SM, Hess WR, Haselkorn R, Joachimiak A. Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria. (2011) Proc Natl Acad Sci U S A. 108:10109-14.
•    Kern J, Wilton R, Zhang R, Binkowski TA, Joachimiak A, Schneewind O. Structure of the SLH domains from Bacillus anthracis surface array protein. (2011) J Biol Chem. 286:26042-9.
•    Kim Y, Tesar C, Mire J, Jedrzejczak R, Binkowski A, Babnigg G,  Sacchettini J, Joachimiak A. Structure of Apo- and Monometalated Forms of NDM-1 – a Highly Potent Carbapenem-Hydrolyzing Metallo-β-Lactamase. (2011) Plos One, 6:e24621.
•    Nusca TD, Kim Y, Maltseva N, Lee JY, Eschenfeldt W, Stols L, Schofield MM, Scaglione JB, Dixon SD, Oves-Costales D, Challis GL, Hanna PC, Pfleger BF, Joachimiak A, Sherman DH. Functional and Structural Analysis of the Siderophore Synthetase AsbB through Reconstitution of the Petrobactin Biosynthetic Pathway from B. anthracis. (2012) J Biol Chem. 287(19):16058-72.
•    Osipiuk J, Mulligan R, Bargassa M, Hamilton JE, Cunningham MA, Joachimiak A. Characterization of Member of DUF1888 Protein Family, Self-cleaving and Self-assembling Endopeptidase. (2012) J Biol Chem. 287(23):19452-61
•    Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A. Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from B. anthracis.  (2012) J Mol Biol. 420(4-5):366-83 
•    Yang DC, Tan K, Joachimiak A, Bernhardt TG.  A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division. (2012) Mol Microbiol. 85(4):768-81.
•    Michalska K, Chang C, Mack JC, Zerbs S, Joachimiak A, Collart FR. Characterization of Transport Proteins for Aromatic Compounds Derived from Lignin: Benzoate Derivative Binding Proteins. (2012) J Mol Biol. 423(4):555-75
•    Binkowski TA, Marino SR, Joachimiak A. Predicting HLA Class I Non-Permissive Amino Acid Residues Substitutions. (2012) PLoS One. 7(8):e41710
•    Kim Y, Cunningham MA, Mire J, Tesar C, Sacchettini J, Joachimiak A. NDM-1, the ultimate promiscuous enzyme: substrate recognition and catalytic mechanism. (2013) FASEB J. 27(5):1917-27
•    Michalska K, Tan K, Li H, Hatzos-Skintges C, Bearden J, Babnigg G, Joachimiak A. GH1 family 6-P-β-glucosidases from human microbiome lactic acid bacteria. (2013) Acta Crystallographica Section D, 69(Pt 3):451-63
•    Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A. (2013) Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets, Proc. Nat. Acad. Sci. USA, 110(19):E1716-23
•    Finfrock YZ, Stern EA, Alkire RW, Kas JJ, Evans-Lutterodt K, Stein A, Duke N, Lazarski K, Joachimiak A. Mitigation of X-ray damage in macromolecular crystallography by submicrometre line focusing. (2013) Acta Crystallogr D Biol Crystallogr. 69(Pt 8):1463-9
•    Lemak S, Beloglazova N, Nocek B, Skarina T, Flick R, Brown G, Popovic A, Joachimiak A, Savchenko A, Yakunin AF. The ​“Ring” of the CRISPR: toroidal structure and nuclease activity of the Cas4 protein of CRISPR interference, (2013) J. Am. Chem. Soc. 135(46):17476-87
•    Tan K, Chhor G, Binkowski TA, Jedrzejczak RP, Makowska-Grzyska M, Joachimiak A. Sensor domain of histidine kinase KinB of Pseudomonas — a helix-swapped dimer, (2014) J. Biol. Chem. 89(18): 12232-44
•    Michalska K, Chhor G, Clancy S, Jedrzejczak R, Babnigg G, Winans SC, Joachimiak A. RsaM: a transcriptional regulator of Burkholderia, with novel fold. (2014) FEBS Letters 281(18):4293-306
•    Lemak S, Nocek B, Beloglazova N, Skarina T, Flick R, Brown G, Joachimiak A, Savchenko A, Yakunin AF. The CRISPR-associated Cas4 protein Pcal_0546 from P. calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity. (2014) Nucleic Acids Res. 42(17):11144-55
•    Sun Z, Khan J, Makowska-Grzyska M, Zhang M, Cho JH, Suebsuwong , Vo ,  Gollapalli DR, Kim Y, Joachimiak A, Hedstrom L, Cuny GD. Synthesis, in vitro evaluation and co-crystal structure of 4-oxo-[1]benzopyrano[4,3-c]pyrazole Cryptosporidium par-vum inosine 5’-monophosphate dehydrogenase (CpIMPDH) inhibitors. (2014) J Medicinal Chemistry. 57(24):10544-50
•    Makowska-Grzyska M, Kim Y, Maltseva N, Osipiuk J, Gu M, Zhang M, Mandapati K, Gollapalli DR, Gorla SK, Hedstrom L, Joachimiak A. A Novel Cofactor Binding Mode in Bacterial IMPDHs Explains Inhibitor Selectivity. (2014) J. Biol. Chem. 290(9):5893-911
•    Hammerstrom TG, Horton LB, Swick MC, Joachimiak A, Osipiuk J, Koehler TM. Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity. (2015) Mol Microbiol.  95(3):426-41
•    Paul S, Aggarwal C, Thakur JK, Bandeppa GS, Khan MA, Pearson LM, Babnigg G, Giometti CS, Joachimiak A. Induction of Osmoadaptive Mechanisms and Modulation of Cellular Physiology Help Bacillus licheniformis Strain SSA 61 Adapt to Salt Stress. (2015) Curr Microbiol. 70(4):610-7
•    Filippova EV, Kuhn ML, Osipiuk J, Kiryukhina O, Joachimiak A, Ballicora MA, Anderson WF. A Novel Polyamine Allosteric Site of SpeG from Vibrio cholerae Is Revealed by Its Dodecameric Structure. (2015) J Mol Biol 427(6ptB):1316-34
•    Yu HY, Ziegelhoffer T, Osipiuk J, Ciesielski SJ, Baranowski M, Zhou M, Joachimiak A, Craig EA. Roles of Intramolecular and Intermolecular Interactions in Functional Regulation of the Hsp70 J-protein Co-Chaperone Sis1. (2015) J Mol Biol. 427(7):1632-43
•    Fan Y, Tan K, Chhor G, Butler EK, Jedrzejczak RP, Missiakas D, Joachimiak A. EsxB, a secreted protein from B. anthracis forms two distinct helical bundles. (2015) Protein Sci. 24(9):1389-400
•    Michalska K, Steen AD, Chhor G, Endres M, Webber AT, Bird J, Lloyd KG, Joachimiak A. New aminopeptidase from ​“microbial dark matter” archaeon. (2015) FASEB J. 29(30):18678-98
•    Glaza P, Osipiuk J, Wenta T, Zurawa-Janicka D, Jarzab M, Lesner A, Banecki B, Skorko-Glonek J, Joachimiak A, Lipinska B. Structural and Functional Analysis of Human HtrA3 Protease and Its Subdomains. (2015) PLoS One 10(6):e0131142
•    Filippova EV, Weigand S, Osipiuk J, Kiryukhina O, Joachimiak A, Anderson WF. Substrate-Induced Allosteric Change in the Quaternary Structure of the Spermidine N-Acetyltransferase SpeG. (2015) J Mol BIol.  427(22):3538-53
•    Lohman JR, Ma M, Osipiuk J, Nocek B, Kim Y, Chang C, Cuff M, Mack J, Bigelow L, Li H, Endres M, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B. Structural and evolutionary relationships of ​“AT-less” type I polyketide synthase ketosynthases. (2015) Proc Natl Acad Sci USA. 112(41):12693-8
•    Chang C, Tesar C, Li X, Kim Y, Rodionov DA, Joachimiak A. A novel transcriptional regulator of L-arabinose utilization in human gut bacteria. (2015) Nucleic Acids Res. 43(21):10546-59
•    Makowska-Grzyska M, Kim Y, Gorla SK, Wei Y, Mandapati K, Zhang M, Maltseva N, Modi G, Boshoff HI, Gu M, Aldrich C, Cuny GD, Hedstrom L, Joachimiak A. Mycobacterium tuberculosis IMPDH in Complexes with Substrates, Products and Antitubercular Compounds. (2015) PLoS One. 10(10):e0138976
•    Tan K, Zhou Q, Cheng B, Zhang Z, Joachimiak A, Tse-Dinh YC. Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I. (2015) Nucleic Acids Res. 43(22):11031-46
•    Michalska K, Tan K, Chang C, Li H, Hatzos-Skintges C, Molitsky M, Alkire R, Joachimiak A. In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID. (2015) J Synchrotron Radiat. 22(Pt 6):1386-95
•    Tan K, Cao N, Cheng B, Joachimiak A, Tse-Dinh YC. Insights from the Structure of Mycobacterium tuberculosis Topoisomerase I with a Novel Protein Fold. (2016) J Mol Biol. 428(1):182-93
•    Manjasetty BA, Halavaty AS, Luan CH, Osipiuk J, Mulligan R, Kwon K, Anderson WF, Joachimiak A. Loop-to-helix transition in the structure of multidrug regulator AcrR at the entrance of the drug-binding cavity. (2016) J Struct Biol. 194(1):18-28
•    Sung N, Lee J, Kim JH, Chang C, Joachimiak A, Lee S, Tsai FT. Mitochondrial Hsp90 is a ligand-activated molecular chaperone coupling ATP binding to dimer closure through a coiled-coil intermediate. (2016) Proc Natl Acad Sci USA. 113(11):2952-7
•    Verduzco-Castro EA, Michalska K, Endres M, Juárez-Vazquez AL, Noda-García L, Chang C, Henry CS, Babnigg G, Joachimiak A, Barona-Gómez F. Co-occurrence of Analogous Enzymes Determines Evolution of a Novel (βα)8-Isomerase Sub-family After Non-conserved Mutations in Flexible Loop. (2016) Biochem J. 473(9):1141-52
•    Kim Y, Joachimiak G, Bigelow L, Babnigg G, Joachimiak A. How Aromatic Compounds Block DNA Binding of HcaR Catabolite Regulator. (2016) J Biol Chem. 291(25):13243-56.
•    Hajighasemi M, Nocek BP, Tchigvintsev A, Brown G, Flick R, Xu X, Cui H, Hai T, Joachimiak A, Golyshin PN, Savchenko A, Edwards EA, Yakunin AF. Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. (2016) Biomacromolecules. 17(6):2027-39
•    Huang L, Khusnutdinova A, Nocek B, Brown G, Xu X, Cui H, Petit P, Flick R, Zallot R, Balmant K, Ziemak MJ, Shanklin J, de Crécy-Lagard V, Fiehn O, Gregory JF 3^rd, Joachimiak A, Savchenko A, Yakunin AF, Hanson AD. A family of metal-dependent phosphatases implicated in metabolite damage-control. (2016) Nature Chem Biol. 12(8):621-7
•    Huang T, Chang CY, Lohman JR, Rudolf JD, Kim Y, Chang C, Yang D, Ma M, Yan X, Crnovcic I, Bigelow L, Clancy S, Bingman CA, Yennamalli RM, Babnigg G, Joachimiak A, Phillips GN, Shen B. Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027. (2016) J Antibiot (Tokyo). 69(10):731-740
•    Rudolf JD, Dong LB, Cao H, Hatzos-Skintges C, Osipiuk J, Endres M, Chang CY, Ma M, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B. Structure of the ent-Copalyl Diphosphate Synthase PtmT2 from Streptomyces platensis CB00739, a Bacterial Type II Diterpene Synthase. (2016) J Am Chem Soc. 138(34): 10905-15
•    Jha RK, Kern TL, Kim Y, Tesar C, Jedrzejczak R, Joachimiak A, and Strauss CEM. A microbial sensor for organophosphate hydrolysis exploiting an engineered specificity switch in a transcription factor. (2016) Nucleic Acids Res. 44(17): 8490-500
•    Popovic A, Hai T, Tchigvintsev A, Hajighasemi M, Nocek B, Khusnutdinova AN, Brown G, Glinos J, Flick R, Skarina T, Chernikova TN, Yim V, Brüls T, Paslier DL, Yakimov MM, Joachimiak A, Ferrer M, Golyshina OV, Savchenko A, Golyshin PN, Yakunin AF. Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families. (2017) Science Rep. 7:44103.
•    Batot G, Michalska K, Ekberg G, Irimpan EM, Joachimiak G, Jedrzejczak R, Babnigg G, Hayes CS, Joachimiak A, Goulding CW. The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamily. (2017) Nucleic Acid Res. 45(9): 5013-5025.
•    Tan K, Deatherage Kaiser BL, Wu R, Cuff M, Fan Y, Bigelow L, Jedrzejczak RP, Adkins JN, Cort JR, Babnigg G, Joachimiak A. Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi. (2017) Protein Sci. 26(9):1738-1748.
•    Forlani G, Nocek B, Chakravarthy S, Joachimiak A. Functional Characterization of Four Putative δ(1)-Pyrroline-5-Carboxylate Reductases from Bacillus subtilis. (2017) Front Microbiol. 8:1442.
•    Wellington S, Nag PP, Michalska K, Johnston SE, Jedrzejczak RP, Kaushik VK, Clatworthy AE, Siddiqi N, McCarren P, Bajrami B, Maltseva NI, Combs S, Fisher SL, Joachimiak A, Schreiber SL, Hung DT. A small-molecule allosteric inhibitor of Mycobacterium tuberculosis tryptophan synthase. (2017) Nat Chem Biol. Sep;13(9):943-950.
•    Michalska K, Gucinski GC, Garza-Sánchez F, Johnson PM, Stols LM, Eschenfeldt WH, Babnigg G, Low DA, Goulding CW, Joachimiak A, Hayes CS. Structure of a novel antibacterial toxin that exploits elongation factor Tu to cleave specific transfer RNAs, (2017) Nucleic Acids Research, 45(17):10306-10320.
•    van den Bosch TJM, Tan K, Joachimiak A, Welte CU. Functional profiling and crystal structures of isothiocyanate hydrolases found in gut-associated and plant pathogenic bacteria. (2018) Appl Environ Microbiol. pii: AEM.00478-18.
•    Tan K, Tesar C, Wilton R, Jedrzejczak RP, Joachimiak A. Interaction of antidiabetic α-glucosidase inhibitors and gut bacteria α-glucosidase. Protein Sci. 2018 27(8):1498-1508.
•    Michalska K, Quan Nhan D, Willett JLE, Stols LM, Eschenfeldt WH, Jones AM, Nguyen JY, Koskiniemi S, Low DA, Goulding CW, Joachimiak A, Hayes CS. Functional plasticity of antibacterial EndoU toxins. Mol Microbiol. 2018 109(4):509-527.
•    Cao N, Tan K, Annamalai T, Joachimiak A, Tse-Dinh YC. Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site. (2018) Nucleic Acids Res. Jun 14.
•    Chang C, Amer BR, Osipiuk J, McConnell SA, Huang IH, Hsieh V, Fu J, Nguyen HH, Muroski J, Flores E, Ogorzalek Loo RR, Loo JA, Putkey JA, Joachimiak A, Das A, Clubb RT, Ton-That H. In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking. (2018) Proc Natl Acad Sci USA. 12;115(24):E5477-E5486.
•    Chang CY, Yan X, Crnovcic I, Annaval T, Chang C, Nocek B, Rudolf JD, Yang D, Hindra, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B. Resistance to Enediyne Antitumor Antibiotics by Sequestration. Cell Chem Biol. 2018 25(9):1075-1085.e4.
•    Kim Y, Chhor G, Tsai CS, Winans JB, Jedrzejczak R, Joachimiak A, Winans SC. Crystal structure of the ligand-binding domain of a LysR-type transcriptional regulator: transcriptional activation via a rotary switch. Mol Microbiol. 2018 110(4):550-561.
•    Dementiev A, Joachimiak A, Nguyen H, Gorelik A, Illes K, Shabani S, Gelsomino M, Ahn EE, Nagar B, Doan N. Molecular mechanism of inhibition of acid ceramidase by carmofur.  (2019) J Med Chem. Jan 24;62(2):987-992
•    Michalska K, Zhang K, March ZM, Hatzos-Skintges C, Pintilie G, Bigelow L, Castellano LM, Miles LJ, Jackrel ME, Chuang E, Jedrzejczak R, Shorter J, Chiu W, Joachimiak A. Structure of Calcarisporiella thermophila Hsp104 disaggregase that antagonizes diverse proteotoxic misfolding events. (2019) Structure Mar 5;27(3):449-463
•    Yu R, Kim Y, Maltseva N, Braunstein P, Joachimiak A, Hedstrom L. Oxanosine monophosphate is a covalent inhibitor of inosine 5′-monophosphate dehydrogenase. (2019) Chem Res Toxicol. Mar 18;32(3):456-466
•    Michalska k, Gale J, Joachimiak G, Chang C, Hatzos-Skintges C, Nocek B, Johnston SE, Bigelow L, Bajrami B, Jedrzejczak RP, Wellington S, Hung DT, Nag P, Fisher SL, Endres M, Joachimiak A. Conservation of the structure and function of bacterial tryptophan synthases. (2019). IUCrJ. May 29;6(Pt 4):649-664
•    Kim Y, Maltseva N, Wilamowski M, Tesar C, Endres M, Joachimiak A. Structural and biochemical analysis of the metallo-β-lactamase L1 from emerging pathogen Stenotrophomonas maltophilia revealed the subtle but distinct di-metal scaffold for catalytic activity. Protein Sci. 2020 Mar;29(3):723-743. Epub 2019 Dec 24. PMID: 31846104.
•    Tan K, Zhou M, Jedrzejczak RP, Wu R, Higuera RA, Borek D, Babnigg G, Joachimiak A. Structures of teixobactin-producing nonribosomal peptide synthetase condensation and adenylation domains, (2020) Current Research in Structural Biology 2, 14-24. PMID: 34235466.
•    Michalska K, Chang C, Maltseva NI, Jedrzejczak R, Robertson GT, Gusovsky F, McCarren P, Schreiber SL, Nag PP, Joachimiak A. Allosteric inhibitors of Mycobacterium tuberculosis tryptophan synthase. Protein Sci. 2020 Mar;29(3):779-788. Epub 2020 Jan 20. PMID: 31930594.
•    Michalska K, Kowiel M, Bigelow L, Endres M, Gilski M, Jaskolski M, Joachimiak A. 3D domain swapping in the TIM barrel of the α subunit of Streptococcus pneumoniae tryptophan synthase. Acta Crystallogr D Struct Biol. 2020 Feb 1;76(Pt 2):166-175. Epub 2020 Jan 31. PMID: 32038047.
•    Osipiuk J, Azizi SA, Dvorkin S, Endres M, Jedrzejczak R, Jones KA, Kang S, Kathayat RS, Kim Y, Lisnyak VG, Maki SL, Nicolaescu V, Taylor CA, Tesar C, Zhang YA, Zhou Z, Randall G, Michalska K, Snyder SA, Dickinson BC, Joachimiak A, Structure of papain-like protease from SARS-CoV-2 and its complexes with non-covalent inhibitors (2020) Nature Commun.12(1):743. PMC7854729
•    Kim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A. Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Protein Sci. 2020 Jul 29(7): 1596-1605. PMID: 32304108.
•    Kneller, DW, Phillips, G, O’Neil, H, Jedrzejczak, R, Stols, S, Joachimiak, A, Coates, L, and Kovalevsky, A. Structural Plasticity of the SARS-CoV-2 3CL Mpro Active Site Cavity Revealed by Room Temperature X-ray Crystallography. Nature Commun. 2020 Jun 24;11(1):3202. PMID: 32581217
•    Guthmiller JJ, Stovicek O, Wang J, Changrob S, Li L, Halfmann P, Zheng NY, Utset H, Stamper CT, Dugan HL, Miller WD, Huang M, Dai YN, Nelson CA, Hall PD, Jansen M, Shanmugarajah K, Donington JS, Krammer F, Fremont D, Joachimiak A, Kawaoka Y, Tesic V, Madariaga ML, Wilson PC. SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike (2021) mBio. Jan 19;12(1):e02940-20. PMID: 33468695
•    Michalska K, Kim Y, Jedrzejczak R, Maltseva NI, Stols L, Endres M, Joachimiak A.  Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes. IUCrJ. 2020 Jul 17;7(Pt 5):814-824. eCollection PMID: 32939273
•    Kneller DW, Phillips G, O’Neill HM, Tan K, Joachimiak A, Coates L, Kovalevsky A. Room-temperature X-ray crystallography reveals the oxidation and reactivity of cysteine residues in SARS-CoV-2 3CL Mpro: insights into enzyme mechanism and drug design. IUCrJ. 2020 Sep 21;7(Pt 6):1028-35. Online ahead of print. PMID: 33063790 
•    March ZM, Sweeney K, Kim H, Yan X, Castellano LM, Jackrel ME, Lin J, Chuang E, Gomes E, Willicott CW, Michalska K, Jedrzejczak RP, Joachimiak A, Caldwell KA, Caldwell GA, Shalem O, Shorter J. Therapeutic genetic variation revealed in diverse Hsp104 homologs. Elife. 2020 Dec 15;9:e57457. Online. PMID: 33319748 
•    Hodge CD, Rosenberg DJ, Grob P, Wilamowski M, Joachimiak A, Hura GL, Hammel M. Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein (2021) MAbs. 13(1):1905978, PMID: 33843452
•    Osipiuk J, Azizi SA, Dvorkin S, Endres M, Jedrzejczak R, Jones KA, Kang S, Kathayat RS, Kim Y, Lisnyak VG, Maki SL, Nicolaescu V, Taylor CA, Tesar C, Zhang YA, Zhou Z, Randall G, Michalska K, Snyder SA, Dickinson BC, Joachimiak A, Structure of papain-like protease from SARS-CoV-2 and its complexes with non-covalent inhibitors (2020) Nature Commun. 2021 Feb 2;12(1):743. PMID: 33531496
•    Kim Y, Wower J, Maltseva N, Chang C, Jedrzejczak R, Wilamowski M, Kang S, Nicolaescu V, Randall G, Michalska K, Joachimiak A. Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2. Commun Biol. 2021 Feb 9;4(1):193. PMID: 33564093
•    Wilamowski M, Sherrell DA, Minasov G, Kim Y, Shuvalova L, Lavens A, Chard R, Maltseva N, Jedrzejczak R, Rosas-Lemus M, Saint N, Foster IT, Michalska K, Satchell KJF, Joachimiak A. 2’-O Methylation of RNA Cap in SARS-CoV-2 captured by serial crystallography, (2021) Proc Natl Acad Sci USA, 18(21):e2100170118. PMID: 33972410
•    Michalska K, Jedrzejczak R, Wower J, Chang C, Baragaña B, Gilbert IH, Forte B, Joachimiak A. Mycobacterium tuberculosis Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation. (2021) Nucleic Acids Res. 49(9):5351-5368. PMID: 33885823. 
•    Michalska K, Joachimiak A. Structural Genomics and the Protein Data Bank. (2021) J Biol Chem. 100747. PMID: 33957120.
•    Dugan HL, Stamper CT, Li L, Changrob S, Asby NW, Halfmann PJ, Zheng NY, Huang M, Shaw DG, Cobb MS, Erickson SA, Guthmiller JJ, Stovicek O, Wang J, Winkler ES, Madariaga ML, Shanmugarajah K, Jansen MO, Amanat F, Stewart I, Utset HA, Huang J, Nelson CA, Dai YN, Hall PD, Jedrzejczak RP, Joachimiak A, Krammer F, Diamond MS, Fremont DH, Kawaoka Y, Wilson PC.Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets.  Immunity. 2021 54(6):1290-1303.e7 PMID: 34022127
•    Wilamowski M, Hammel M, Leite W, Zhang Q, Kim Y, Weiss KL, Jedrzejczak R, Rosenberg DJ, Fan Y, Wower J, Bierma JC, Sarker AH, Tsutakawa SE, Pingali SV, O’Neill HM, Joachimiak A, Hura GL. Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in SARS-CoV-2 replication.  Biophys J. 2021 S0006-3495(21)00491-4. PMID: 34197805 
•    Michalska K, Wellington S, Maltseva N, Jedrzejczak R, Selem-Mojica N, Rosas-Becerra LR, Barona-Gómez F, Hung DT, Joachimiak A. Catalytically impaired TrpA subunit of tryptophan synthase from Chlamydia trachomatis is an allosteric regulator of TrpB.  Protein Sci. 2021 PMID: 34107106 
•    Drayman N, DeMarco JK, Jones KA, Azizi SA, Froggatt HM, Tan K, Maltseva NI, Chen S, Nicolaescu V, Dvorkin S, Furlong K, Kathayat RS, Firpo MR, Mastrodomenico V, Bruce EA, Schmidt MM, Jedrzejczak R, Muñoz-Alía MÁ, Schuster B, Nair V, Han KY, O’Brien A, Tomatsidou A, Meyer B, Vignuzzi M, Missiakas D, Botten JW, Brooke CB, Lee H, Baker SC, Mounce BC, Heaton NS, Severson WE, Palmer KE, Dickinson BC, Joachimiak A, Randall G, Tay S. Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2. Science. 2021 eabg5827. PMID: 34285133
•    Abergel, R. J., Adams, P, Antonopoulos, D, Babnigg, G, Baker, S, Bedinger, D, Borucki, M, Bradfute, S.B., Bunt, T., Daum, C., Fitch, J. P., Gerbasi, V, Gilna, P, Graham, D, Guarnieri, M, Hall, S, Hillson, N, Hong-Geller, E, H, Greg, Jaing, C, Jha, R Kumar, Joachimiak, A, Kamel, Bi, Kimbrel, J, Lillo, A M, Mangadu, B, Meagher, R, Moon, J, Mouncey, N, Morrison, M, Mulakken, Nisha, Nguyen, Hau Thi Bich, Nilsen-Hamilton, Marit, Omberg, Kristin, O’Neal, Hugh, Parks, J, Pasa-Tolic, L, Pennacchio, C, Rakestraw, D, Retterer, S, Salit, M, Simmons, B, Singh, A, Sustarich, J, Thissen, J, Velappan, N, Waldo, G S., Williams, K, Wilson, J, Ye, C, Young, M, Yoshinaga, Yuko, and Zhou, M. COVID-19 Testing R&D Final Report. USA: N. p., 2021. Web. 
•    Fernández-Lucas J, Acebrón I, Wu RY, Alfaro Y, Acosta J, Kaminski PA, Arroyo M, Joachimiak A, Nocek BP, De la Mata I, Mancheño JM. Biochemical and structural studies of two tetrameric nucleoside 2′-deoxyribosyltransferases from psychrophilic and mesophilic bacteria: Insights into cold-adaptation. (2021) Int J Biol Macromol. 192:138-150. PMID: 34624379
•    Lemak S, Serbanescu MA, Khusnutdinova AN, Ruszkowski M, Beloglazova N, Xu X, Brown G, Cui H, Tan K, Joachimiak A, Cvitkovitch DG, Savchenko A, Yakunin AF. Structural and biochemical insights into CRISPR RNA processing by the Cas5c ribonuclease SMU1763 from Streptococcus mutans. (2021) J Biol Chem. 297(5):101251. PMID: 34592310
•    Modi G, Marqus GM, Vippila MR, Gollapalli DR, Kim Y, Manna AC, Chacko S, Maltseva N, Wang X, Cullinane RT, Zhang Y, Kotler JLM, Kuzmic P, Zhang M, Lawson AP, Joachimiak A, Cheung A, Snider BB, Rothstein DM, Cuny GD, Hedstrom L. The Enzymatic Activity of Inosine 5′-Monophosphate Dehydrogenase May Not Be a Vulnerable Target for Staphylococcus aureus Infections. (2021) ACS Infect Dis. PMID: 34590817
•    Alexander LT, Lepore R, Kryshtafovych A, Adamopoulos A, Alahuhta M, Arvin AM, Bomble YJ, Böttcher B, Breyton C, Chiarini V, Chinnam NB, Chiu W, Fidelis K, Grinter R, Gupta GD, Hartmann MD, Hayes CS, Heidebrecht T, Ilari A, Joachimiak A, Kim Y, Linares R, Lovering AL, Lunin VV, Lupas AN, Makbul C, Michalska K, Moult J, Mukherjee PK, Nutt WS, Oliver SL, Perrakis A, Stols L, Tainer JA, Topf M, Tsutakawa SE, Valdivia-Delgado M, Schwede T. Target highlights in CASP14: Analysis of models by structure providers. (2021) Proteins. PMID: 34561912
•    Moiani D, Link TM, Brosey CA, Katsonis P, Lichtarge O, Kim Y, Joachimiak A, Ma Z, Kim IK, Ahmed Z, Jones DE, Tsutakawa SE, Tainer JA. An efficient chemical screening method for structure-based inhibitors to nucleic acid enzymes targeting the DNA repair-replication interface and SARS CoV-2. (2021) Methods Enzymol. 2021;661:407-431. PMID: 34776222
•    Chacko S, Boshoff HIM, Singh V, Ferraris DM, Gollapalli DR, Zhang M, Lawson AP, Pepi MJ, Joachimiak A, Rizzi M, Mizrahi V, Cuny GD, Hedstrom L. Expanding Benzoxazole-Based Inosine 5′-Monophosphate Dehydrogenase (IMPDH) Inhibitor Structure-Activity As Potential Antituberculosis Agents. (2021) J Med Chem. 64(24):18233-18234. PMID: 34855408
•    Li L, Dugan HL, Stamper CT, Lan LY, Asby NW, Knight M, Stovicek O, Zheng NY, Madariaga ML, Shanmugarajah K, Jansen MO, Changrob S, Utset HA, Henry C, Nelson C, Jedrzejczak RP, Fremont DH, Joachimiak A, Krammer F, Huang J, Khan AA, Wilson PC. Improved integration of single-cell transcriptome and surface protein expression by LinQ-View. (2021) Cell Rep Methods. 1(4):100056. PMID: 35475142
•    Mullowney MW, Maltseva NI, Endres M, Kim Y, Joachimiak A, Crofts TS. Functional and Structural Characterization of Diverse NfsB Chloramphenicol Reductase Enzymes from Human Pathogens. (2022) Microbiol Spectr. 2022 Feb 23;10(2):e0013922. ]PMID: 35195438
•    Herrera A, Kim Y, Chen J, Jedrzejczak R, Shukla S, Maltseva N, Joachimiak G, Welk L, Wiersum G, Jaroszewski L, Godzik A, Joachimiak A, Satchell KJF. A Genomic Island of Vibrio cholerae Encodes a Three-Component Cytotoxin with Monomer and Protomer Forms Structurally Similar to Alpha-Pore-Forming Toxins. (2022) J Bacteriol. :e0055521. PMID: 35435721
•    St John FJ, Crooks C, Kim Y, Tan K, Joachimiak A. The first crystal structure of a xylobiose-bound xylobiohydrolase with high functional specificity from the bacterial glycoside hydrolase family 30, subfamily 10. (2022) FEBS Lett. PMID: 35876256
•    Babnigg G, Sherrell D, Kim Y, Johnson JL, Nocek B, Tan K, Axford D, Li H, Bigelow L, Welk L, Endres M, Owen RL, Joachimiak A. Data collection from crystals grown, PMID: 35916224
•    Bartelli NL, Passanisi VJ, Michalska K, Song K, Nhan DQ, Zhou H, Cuthbert BJ, Stols LM, Eschenfeldt WH, Wilson NG, Basra JS, Cortes R, Noorsher Z, Gabraiel Y, Poonen-Honig I, Seacord EC, Goulding CW, Low DA, Joachimiak A, Dahlquist FW, Hayes CS. Proteolytic processing induces a conformational switch required for antibacterial toxin delivery. (2022) Nat Commun. 13(1):5078. PMID: 36038560 
•    Sherrell DA, Lavens A, Wilamowski M, Kim Y, Chard R, Lazarski K, Rosenbaum G, Vescovi R, Johnson JL, Akins C, Chang C, Michalska K, Babnigg G, Foster I, Joachimiak A. Fixed-target serial crystallography at the Structural Biology Center. (2022) J Synchrotron Radiat. 29(Pt 5):1141-1151. PMID: 36073872
•    Wilamowski M, Sherrell DA, Kim Y, Lavens A, Henning RW, Lazarski K, Shigemoto A, Endres M, Maltseva N, Babnigg G, Burdette SC, Srajer V, Joachimiak A. Time-resolved β-lactam cleavage by L1 metallo-β-lactamase. (2022) Nature Commun. 13(1):7379. PMID: 36450742
•    Inniss NL, Kochan TJ, Minasov G, Wawrzak Z, Chang C, Tan K, Shuvalova L, Kiryukhina O, Pshenychnyi S, Wu R, Dubrovska I, Babnigg G, Endres M, Anderson WF, Hauser AR, Joachimiak A, Satchell KJF. A Structural Systems Biology Approach to High-Risk CG23 Klebsiella pneumoniae. (2023) Microbiol Resour Announc. 12(2):e0101322. PMID: 36695589
•    Reardon-Robinson ME, Nguyen MT, Sanchez BC, Osipiuk J, Rückert C, Chang C, Chen B, Nagvekar R, Joachimiak A, Tauch A, Das A, Ton-That H. A cryptic oxidoreductase safeguards oxidative protein folding in Corynebacterium diphtheriae. (2023) Proc Natl Acad Sci U S A. 21;120(8):e2208675120. PMID: 36787356
•    Cheng, J.X., Wood, S., Tang, D., Degenstein, L., Joachimiak, A. and Stevens, R., (2023). Overcoming Venetoclax Resistance in Leukemia with AI-Designed RNA Epigenetic Inhibitors. Blood, 142 (Supplement 1), pp.5738-5738.
•    Sanders BC, Pokhrel S, Labbe AD, Mathews II, Cooper CJ, Davidson RB, Phillips G, Weiss KL, Zhang Q, O’Neill H, Kaur M, Schmidt JG, Reichard W, Surendranathan S, Parvathareddy J, Phillips L, Rainville C, Sterner DE, Kumaran D, Andi B, Babnigg G, Moriarty NW, Adams PD, Joachimiak A, Hurst BL, Kumar S, Butt TR, Jonsson CB, Ferrins L, Wakatsuki S, Galanie S, Head MS, Parks JM. Potent and selective covalent inhibition of the papain-like protease from SARS-CoV-2. (2023) Nature Commun. 14(1):1733. PMID: 36977673
•    Wydorski PM, Osipiuk J, Lanham BT, Tesar C, Endres M, Engle E, Jedrzejczak R, Mullapudi V, Michalska K, Fidelis K, Fushman D, Joachimiak A, Joachimiak LA. Dual domain recognition determines SARS-CoV-2 selectivity for human ISG15 and K48-linked di-ubiquitin. (2023) Nature Commun. 14(1):2366. PMID: 37185902
•    Witkowska M, Jedrzejczak RP, Joachimiak A, Cavdar O, Malankowska A, Skowron PM, Zylicz-Stachula A. Promising approaches for the assembly of the catalytically active, recombinant Desulfomicrobium baculatum hydrogenase with substitutions at the active site. (2023) Microb Cell Fact. 22(1):134. PMID: 37479997
•    Dementiev A, Lillington SP, Jin S, Kim Y, Jedrzejczak R, Michalska K, Joachimiak A, O’Malley MA. Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis. (2023) Appl Microbiol Biotechnol. PMID: 37548665
•    Chang C, Ton-That H, Osipiuk J, Joachimiak A, Das A, Ton-That H. Molecular basis for dual functions in pilus assembly modulated by the lid of a pilus-specific sortase, (2024) J Biol Chem. 107329. Online ahead of print. PMID: 38679328
•    Rosas-Lemus M, Dey S, Minasov G, Tan K, Anderson SM, Brunzelle J, Nocadello S, Shabalin I, Filippova E, Halavaty A, Kim Y, Maltseva N, Osipiuk J, Minor W, Joachimiak A, Savchenko A, Anderson WF, Satchell KJF; A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile. Microbiol Resour Announc. (2023) 12(10):e0050723. PMID: 37747257
•    Jadhav, P, Huang, B, Osipiuk, J, Zhang, X, Tan, H, Tesar, C, Endres, M, Jedrzejczak, R, Deng, X, Joachimiak, A, Cai, J, Wanga, J, Structure-based design of SARS-CoV-2 papain-like protease inhibitors (2024) European Journal of Medicinal Chemistry, 264:116011 PMID: 38065031
•    Kim Y, Maltseva N, Tesar C, Jedrzejczak R, Endres M, Ma H, Dugan HL, Stampe CT, Chang C, Li L, Changrob S, Zheng NY, Huang M, Ramanathan A, Wilson P, Michalska K, and Joachimiak A, Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human mAbs, (2024) iScience, 27(2):108976. PMID: 38327783
•    Thibert, S, Reid, D, Wilson, J, Varikoti, R, Maltseva, N, Schultz, K, Kruel, A, Babnigg, G, Joachimiak, A, Kumar, N, Zhou, M, Native Mass Spectrometry Dissects the Structural Dynamics of an Allosteric Heterodimer of SARS-CoV-2 Nonstructural Proteins, (2024) J Am Soc Mass Spectrom. Online PMID: 38535992
•    Lawson CL, Kryshtafovych A, Pintilie GD, Burley SK, Černý J, Chen VB, Emsley P, Gobbi A, Joachimiak A, Noreng S, Prisant MG, Read RJ, Richardson JS, Rohou AL, Schneider B, Sellers BD, Shao C, Sourial E, Williams CI, Williams CJ, Yang Y, Abbaraju V, Afonine PV, Baker ML, Bond PS, Blundell TL, Burnley T, Campbell A, Cao R, Cheng J, Chojnowski G, Cowtan KD, DiMaio F, Esmaeeli R, Giri N, Grubmüller H, Hoh SW, Hou J, Hryc CF, Hunte C, Igaev M, Joseph AP, Kao WC, Kihara D, Kumar D, Lang L, Lin S, Maddhuri Venkata Subramaniya SR, Mittal S, Mondal A, Moriarty NW, Muenks A, Murshudov GN, Nicholls RA, Olek M, Palmer CM, Perez A, Pohjolainen E, Pothula KR, Rowley CN, Sarkar D, Schäfer LU, Schlicksup CJ, Schröder GF, Shekhar M, Si D, Singharoy A, Sobolev OV, Terashi G, Vaiana AC, Vedithi SC, Verburgt J, Wang X, Warshamanage R, Winn MD, Weyand S, Yamashita K, Zhao M, Schmid MF, Berman HM, Chiu W., Outcomes of the EMDataResource cryo-EM Ligand Modeling Challenge. Nature Methods. 2024 Jul;21(7):1340-1348. Epub 2024 Jun 25. PMID: 38918604
•    Kelley EH, Osipiuk J, Korbas M, Endres M, Bland A, Ehrman V, Joachimiak A, Olsen KW, Becker DP. N α -acetyl-L-ornithine deacetylase from Escherichia coli and a ninhydrin-based assay to enable inhibitor identification. Front Chem. 2024 Jul 11;12:1415644. 2024.1415644. eCollection 2024. PMID: 39055043
•    Kim Y, Lee SH, Gade P, Nattermann M, Maltseva N, Endres M, Chen J, Wichmann P, Hu Y, Marchal DG, Yoshikuni Y, Erb TJ, Gonzalez R, Michalska K, Joachimiak A. Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family. Commun Chem. 2024 Jul 21;7(1):160. PMID: 39034323 
•    Chen JCH, Gilski M, Chang C, Borek D, Rosenbaum G, Lavens A, Otwinowski Z, Kubicki M, Dauter Z, Jaskolski M, Joachimiak A. Solvent organization in the ultrahigh-resolution crystal structure of crambin at room temperature. IUCrJ. 2024 Sep 1;11(Pt 5):649-663. PMID: 39190507